Extended Data Fig. 1: Generation and validation of EEC deficient mice.
From: Interaction between the gut microbiota and colonic enteroendocrine cells regulates host metabolism
(a) Schematic representation of the colonic EEC deficiency model. Created with BioRender.com. (b) qRT-PCR of Neurog3 gene along regions of the mouse bowel. Values are expressed as fold of the average of wild-type animals in the duodenum. Statistics derived from biological replicates (8 weeks of age, n = 3 per group). (c) Morphometric quantification of chromogranin A (CHGA) labeled cells along the intestinal epithelium. At least 10 confocal-acquired immunofluorescence images from each intestinal segment (duodenum, jejunum, ileum, cecum, proximal colon, distal colon) from 3 adult mice of either genotype were used for morphometric analysis (mice of 8 weeks of age). Chromogranin A (CHGA) positive cells were visually counted and normalized to the number of cells per 10 intestinal crypts. (d) Representative confocal images of immunofluorescent stained regions of the mouse intestine. (e) Representative light microscopy images of HE and Alcian blue stained regions of the mouse intestine (one representative animal in each group, n > 3 per group). Mean expression values are represented by bars and individual values by jitter plots. Error bars are ± s.e.m. Two-tailed unpaired t-test used for all pairwise comparisons.
