Extended Data Fig. 3: Supportive Data to Main Fig. 3.
From: A tumor-derived type III collagen-rich ECM niche regulates tumor cell dormancy
All numerical data are presented as mean +/−SEM. (a) Representative images of Masson’s Trichrome from T-HEp3 mice tumors with or without type III collagen co-injection. Scale bar, 50μm. (b) Normalized distribution of collagen fiber orientation from tumors presented in A (n = 5 independent tumors per group, 2 images analyzed per tumors). Cumulative distributions were compared using an unpaired two-tailed Kolmogorov Smirnov test with 95% confidence level. (c) Tumor growth of D2.A1 +/− type III collagen co-injection (n = 5 mice per group). Curves were compared using a two-way ANOVA with mixed model effects analysis and a Bonferroni correction and a 95% confidence interval. (d) Tumor growth of 4T1 +/− type III collagen co-injection (n = 5 mice per group). Curves were compared using a two-way ANOVA with mixed model effects analysis and a Bonferroni correction and a 95% confidence interval. (e) FACS analysis for percentage of T-HEp3 live cells (green), dead cells (red), apoptotic cells(yellow) and necrotic cells (orange) plated on plastic, type I collagen, or type III collagen matrix (n = 3 independent experiments). Distributions were compared using a Chi-squared test with 95% confidence interval. (f) Time points from an 18hrs time lapse movie of D-HEp3 plated on type I or III collagen. (t=hours). Scale bar, 10 μm. Related to Supplemental Movies 3 and 4. (g) APOTOX assay of T-HEp3 plated on different concentrations of type III collagen for 24hrs (n = 3 independent experiments).
