Extended Data Fig. 3: LEC-like cell abundance is correlated with patient survival.
a-b, Gating strategies for FACS analysis shown in Fig. 2a,b. c, Representative images of immunohistochemical staining of pan-endothelial cell maker CD31 and LEC marker Podoplanin in non-neoplastic brain tissues and glioblastoma tissues. Glioblastoma tissue fractions exhibit enriched LECs. Scale bars, 50 μm. Images are representative of three independent experiments. d, Two-tailed Spearman correlation between HBEC cells and GBM patient 25-derived BECs using proteomics data. e, Two-tailed Spearman correlation between HBEC cells and glioblastoma patient 26-derived BECs using proteomics data. f, Spearman correlation between HBE-5i cells and glioblastoma patient-derived BECs using SMART-seq data. g, Spearman correlation between commercial LECs and glioblastoma patient-derived LECs using SMART-seq data. h, FACS plots demonstrating isolation of LEC-like cells from GL261 glioma cells. Cells from mouse tumor tissue were stained with CD45-APC-Cy7, CD31-PE, and Podoplanin-FITC.
