Extended Data Fig. 7: Dynamics of aPD1 therapy with 4-1BB agonist. Related to Fig. 5.
(a) Heatmaps showing per mouse (rows) the percentage of Tot1 cells in bins across the trajectories in the tumor with a4-1BB + aPD1 combination treatment at day 6 after adoptive transfer. (b) Means across mice of the percentage of Tot1 cells in bins of the inferred trajectories, comparing combination treated mice (colored) vs. treatment naïve (grey circles) and aPD1 treated (grey triangles) mice, at day 6 post transfer for the combination and time-corresponding stages 3 and 4 from Fig. 2e. (c) Bar graphs of the down-sampled UMI log-fold change of a4-1BB + aPD1 vs. control pooled Tot1 cells, after controlling for distributional shifts across the inferred trajectories. Genes with at least 0.7 log fold change in one or more trajectories at day 6 post transfer are shown. Positive values are higher in combination treated cells.
