Extended Data Fig. 2: Temporal dynamics of CD8+ T cell in cancer. Related to Fig. 2.
(a) FACS plots of TIM3+ versus PD1+ expression in Tot1, Tbystander, and Tendo at day 5 after adoptive transfer (day 15 post tumor inoculation) in the B16-OVA TME. Error bars represent the standard error measurement SEM. Statistics are based on one-way ANOVA with Tukey’s post hoc test. ***P < 0.0006. (b) Means across mice of the percentage of Tot1 (colored by trajectory) or Tbystander (green) cells in bins of the inferred trajectories, at days 3 and 6 post adoptive transfer. (c) Means per mouse of the fraction of Tot1 cells active for the different functional programs (Y-axis), with mice orders by the defined stages of the dynamics. Each black dot is a mouse, and curves represent the smoothed trends across stages. (d) Means across Tot1 cells per mouse of genes’ normalized expression (Y-axis), with mice orders by the defined stages of the dynamics. Each black dot is a mouse, and curves represent the smoothed gene expression across stages.
