Extended Data Fig. 3: Characterization of murine CAR T cells sourced from either pmel-1 or C57BL6/J mice.
(a) Staining of indicated murine T cell population with activation markers CD25 and CD69 following co-incubation with ST- or VHH-expressing E0771 tumor cells. Data is representative of 3 technical replicates from a single experiment. (b) IFN-γ secretion by Pmel-1 or C57BL/6J derived αVHH CAR T cells quantified following a 24 hr coculture with MC38-VHH tumor cells at a 1:1 E:T ratio. Mean of n = 3 technical replicates from a single experiment. (c) Cytotoxicity of Pmel-1 and C57BL/6 derived αVHH CAR T cells were assessed following a 24 hr coculture with MC38-VHH at a 1:1 E:T ratio. Mean of n = 3 technical replicates from a single experiment.
