Extended Data Fig. 8: The mild seasonal influenza A (H1N1) did not cause a senescence-like phenotype.
a–d. Syrian hamsters were intranasally inoculated with SARS-CoV-2 (B.1.1.7) 5.6 × 105 PFU (in 80 μL), medium (mock) as described in Fig. 4, or influenza A (H1N1) pdm09 5.6 × 10^4 PFU (80 μL). Timeline of experiment (a). The body weight were monitored until day 14 (mock; n = 6, CoV2; n = 8, H1N1; n = 7) (b). Syrian hamsters were infected with SARS-CoV-2 (CoV2) (n = 3), mock (n = 3), or H1N1 (n = 6) and euthanized on day 3 post-infection. The H1N1 infection was monitored by RT-qPCR (c). The hamsters infected with these viruses were euthanized on day 14 post-infection (mock; n = 11, CoV2; n = 8, H1N1; n = 7), and were subjected to RT-qPCR analysis for the expression of senescence marker (p16INK4a) and SASP factors gene (d). The data for mock and SARS-CoV-2-infected hamsters are the same as those used in Fig. 4a–e. For all graphs, error bars indicate mean ± standard deviation (s.d.). Statistical significance was determined with one-way ANOVA followed by Tukey multiple comparison tests in (d).
