Extended Data Fig. 3: Genetic or chemical inhibition of AHCY alleviates oncogene-induced senescence.
a, Left, representative images of immunofluorescence staining for AHCY (red). Scale bar, 50 μm. Right, single-cell intensities for AHCY (n = 1,000 cells per condition). The statistical significance was calculated using unpaired two-tailed t-test. b, Left, representative images of immunofluorescence staining for γH2AX (red). Right, quantification of immunofluorescence staining for γH2AX (n = 4). c, Quantification of immunofluorescence staining for BrdU of IMR90 ER:RAS cells four days after treatment with 4OHT or vehicle (DMSO) and 2.5 μM DZNep, 10 μM D-eritadenine and 4 μM TGF-β RI kinase inhibitor II as positive control. d, Expression levels of AHCY (n = 4). e, Expression levels of INK4a (encoding for p16INK4a, n = 4). f, Principal component analysis (PCA) for the experiment described in Fig. 3f. g,h, GSEA signatures from the same experiment. All statistical significances were calculated using one-way ANOVA. All error bars represent mean ± s.d; n represents independent experiments unless otherwise stated.
