Fig. 1: Transcriptional profiling by bulk RNA-seq to investigate the sex-dependent microglial aging process and ADEM gene set.
From: Transcriptional and epigenetic decoding of the microglial aging process
a, Scheme of timepoints for microglia bulk RNA-seq analysis. Microglia were collected by FACS from C57BL/6J female and male mouse brains. b,c, Heat maps of microglial DEGs during the aging process in female (b) and male (c) mice. Cells are colored according to the z-score, and stages are separated by gap. d,e, PCA plots of microglial phenotypes during the aging process in female (d) and male (e) mice. f, Scatter plot showing linear regression of representative genes correlated with the aging process. Gray shading represents the 95% confidence interval, and Pearson’s correlation coefficients and P values are shown. g,h, Heat maps of ADEM genes during the aging process in female (g) and male (h) mice. Cells are colored according to the z-score, and stages are separated by gap. i, Top 10 significantly enriched BPs of ADEM genes annotated by GO (q < 0.05). j, Top 10 significantly enriched canonical pathways of ADEM genes annotated by IPA (q < 0.05). k, Venn diagram revealing 21 genes overlapping between the ADEM and DAM gene sets. Genes in both ADEM and DAM gene sets are listed below. l, Scatter plot showing linear regression of log2FC of overlapping genes between ADEM and DAM gene sets, revealing that they are positively correlated. Gray shading represents the 95% confidence interval, and Pearson’s correlation coefficient and P value are shown at the bottom. m, Venn plot revealing that only three genes are overlapping between ADEM and ARM gene sets. Genes in both ADEM and ARM gene sets are listed below. n = 2–6 mice for each group. BP, biological process; F, female; F03_1, 3-month-old female, biological replicate 1; M03_1, 3-month-old male, biological replicate 1, and so on in a similar fashion; M, male; MO, months old; Dim, dimension.
