Extended Data Fig. 1: Proteomic analysis of aged and young monkey hearts.
From: SIRT2 counteracts primate cardiac aging via deacetylation of STAT3 that silences CDKN2B
a, Box plot showing the normalized intensities for each replicate in proteomics of young and aged monkey hearts. The boxes show the median values (centre line) and interquartile ranges. The whiskers extend from the quartile to the minimum and maximum values. b, Bar plot showing the distribution of the number of peptides in identified proteins of proteomics of young and aged monkey hearts. c, Heatmap showing the Euclidean distance for proteomic data between young and aged monkey hearts. The color key of the Euclidean distance from blue to white indicates strong to weak correlations. d, Principal component analysis of proteomic data between young and aged monkey hearts. e, Heatmap showing the DEPs overlapped with the epigenetic regulation-associated genes in aged monkey hearts. The color key from blue to purple indicates log2(fold change) from low to high. f, Left, box plot showing the protein level of SIRT2 in young and aged female monkey hearts. Right, box plot showing the protein level of SIRT2 in young and aged male monkey hearts. The boxes show the median values (centre line) and interquartile ranges. The whiskers extend from the quartile to the minimum and maximum values. Two-tailed Student’s t-test, n = 3 replicates per group. g, Western blotting of SIRT2 in young and aged female or male monkey hearts. Data are presented as the mean ± s.e.m., two-tailed Student’s t-test. n = 4 biological replicates. h, Network plot showing DEPs that are common to the aged monkey hearts and plasma of aging individuals or patients with cardiovascular diseases. Node colors from blue to purple indicate log2(fold change) from low to high. i, Point plot showing DEPs that have not been annotated with four aging-related cardiovascular diseases. Point size indicates absolute log2(fold change).
