Extended Data Fig. 3: Supplement of gene expression in different cell types changed throughout human ovarian aging.
From: Spatiotemporal transcriptomic changes of human ovarian aging and the regulatory role of FOXP1
(a) Heatmaps showing the upregulated (left) and downregulated (right) DEGs of each cell type between the old and young groups (O/Y), middle and young groups (M/Y), and old and middle groups (O/M). Gene numbers on the left represents from top to bottom, DEGs shared by at least two cell types, DEGs shared by at least two groups, unique DEGs of each cell type in each group. The numbers of unique DEGs are annotated. (b) Venn diagram of DEGs shared by three groups. Y, young; M, middle; O, old. (c) Fluorescence-based-β-Gal staining of human ovaries from young, middle and old group. The scores are listed on the right. Data are presented as the mean ± SEM. n = 10 for each group (one-way ANOVA). (d) Relative mRNA expression of SASPs in human ovaries at different ages by RT–qPCR. Data are presented as the mean ± SEM. n = 9 for each group (one-way ANOVA). (e) Gene set score analysis of NF-κB signaling pathway in eight ovarian cell types of different groups. Two-sided Wilcoxon rank-sum tests. Box-and-whisker plots (minimum, 25th percentile, median, 75th percentile, maximum). n = 3 per age group. (f) Protein expression of cellular senescence-related genes in human ovaries detected by Western blot. Representative images were shown. Data are presented as the mean ± SEM. This test was repeated three times (one-way ANOVA).
