Fig. 5: Spatiotemporal changes of five T&S cells subpopulations during aging.
From: Spatiotemporal transcriptomic changes of human ovarian aging and the regulatory role of FOXP1
a, UMAP visualization of T&S cell subclusters. b, Dot plot heat map showing top T&S cell subclusters markers. c, Heat map showing the highly expressed genes and GO terms specifically in T&S subtype cells. d, The spatial cluster distribution of each subclusters (left) and ST spot overlapped with H&E staining (right). e, IHC for markers of T&S subtype cells. The experiment was repeated three times. f, Pseudotime-ordered analysis of T&S cells (left). Subtypes are labeled by colors (right). g, A 2D graph of the pseudotime-ordered T&S cells from the young, middle and older age groups. The cell density distribution is shown in the above figure. h, Gene set score analysis of cellular senescence pathways in T&S subtype cells with age. Data were analyzed by two-sided Wilcoxon rank-sum tests. Box-and-whisker plots show the minimum, 25th percentile, median, 75th percentile and maximum. n = 3 per age group. i, Violin plot showing the expression of CDKN1A in five T&S cells subclusters. Data were analyzed by two-sided Wilcoxon rank-sum tests. j, ST spot overlay of CDKN1A expression (left) and representative images of CDKN1A expression by IHC (right) in T&S cells of three groups. k, ST spot overlay of SASPs (HMGA1, PIM1 and CAV1) expression in T&S cells of three groups. The ST data analysis is shown on the right. Data were analyzed by two-sided Wilcoxon rank-sum tests. The analysis was conducted in each ovarian tissue (n = 15). l, The correlation analysis of SASPs levels with age in pT&S cells from human ovaries. n = 25 (Pearson correlation analysis, two-sided).
