Fig. 7: Conditional knockout of FOXP1 in GCs accelerates ovarian aging in mice.
From: Spatiotemporal transcriptomic changes of human ovarian aging and the regulatory role of FOXP1
a, Schematic representation of the deletion of FOXP1 in GCs by using CYP19A1-Cre transgenic mice. b, Ovaries of FOXP1+/+ and FOXP1tr/tr mice at 12 weeks. c, H&E staining of ovaries from FOXP1+/+ and FOXP1tr/tr mice. d, Comparison of follicle numbers of FOXP1+/+ and FOXP1tr/tr mice. Data are presented as the mean ± s.e.m. n = 5 for each group (unpaired two-tailed t-test). e, Serum AMH level of FOXP1+/+ and FOXP1tr/tr mice. Data are presented as the mean ± s.e.m. n = 5 for each group (unpaired two-tailed t-test). f, Serum E2 level of FOXP1+/+ and FOXP1tr/tr mice. Data are presented as the mean ± s.e.m. n = 5 for each group (unpaired two-tailed t-test). g, Litter size of mated mice. Data are presented as the mean ± s.e.m. n = 5 for each group (unpaired two-tailed t-test). h, SA-β-gal staining of ovaries from FOXP1+/+ and FOXP1tr/tr mice. The number of SA-β-gal-positive cells was shown on the right. Data are presented as the mean ± s.e.m. n = 5 for each group (unpaired two-tailed t-test). i, Relative RNA expression of SASPs in GCs from FOXP1+/+ and FOXP1tr/tr mice. Data are presented as the mean ± s.e.m. n = 3 for each group (unpaired two-tailed t-test). j, Representative images of γH2AX staining in ovaries of FOXP1+/+ and FOXP1tr/tr mice. Data are presented as the mean ± s.e.m. n = 5 for each group (unpaired two-tailed t-test). k, TUNEL staining of ovaries from FOXP1+/+ and FOXP1tr/tr mice. Data are presented as the mean ± s.e.m. n = 5 for each group (unpaired two-tailed t-test).
