Fig. 8: Quercetin treatment protects the ovarian reserve in middle-aged mice.
From: Spatiotemporal transcriptomic changes of human ovarian aging and the regulatory role of FOXP1
a, SA-β-gal staining in COV434 upon administration of fisetin (F), quercetin (Q) and dasatinib (D) in cells with knockdown of FOXP1. Data are presented as the mean ± s.e.m. n = 5 for each group (unpaired two-tailed t-test). b, Relative RNA expression of CDKN1A in si-FOXP1 COV434 treated with F, Q and D. Data are presented as the mean ± s.e.m. n = 3 for each group (unpaired two-tailed t-test). c, EdU incorporation assay of COV434 upon administration of F, Q and D in cells with knockdown of FOXP1. Data are presented as the mean ± s.e.m. n = 5 for each group (unpaired two-tailed t-test). d, FOXP1 protein expression in COV434 treated by F, Q and D. This test was repeated three times. Data are presented as the mean ± s.e.m. (one-way ANOVA). n = 3 for each group. e, Experimental design to test the effects of quercetin on ovarian aging. f, Estrous cycles monitoring of mice. n = 25 for each group (Fisher Freeman Halton, two-sided). g, Serum AMH levels. Data are presented as the mean ± s.e.m. n = 8 for each group (unpaired two-tailed t-test). h, Follicle counting results according to ovary serial sections. ANF, antral follicle; ATF, atretic follicle; PMF, primordial follicle; PF, primary follicle; SF, secondary follicle; CL, corpus luteum. Data are presented as the mean ± s.d. n = 6 for each group (unpaired two-tailed t-test). i, The proportion of successful pregnant mice. n = 8 for each group (chi-squared test, two-sided). j, SA-β-gal staining of mice ovaries. k, Statistical analysis of SA-β-gal-positive GCs in mice ovaries. Data are presented as the mean ± s.e.m. (one-way ANOVA). n = 5 for each group. l, Western blot analysis of FOXP1, CDKN1A and γH2AX levels from the ovaries of treated mice. m, Densitometry quantified data of western blot analysis. Data are presented as the mean ± s.e.m. (unpaired two-tailed t-test). This test was repeated three times. n, Schematic illustration showing that downregulation of FOXP1 in GCs and T&S cells contributes to ovarian senescence.
