Extended Data Fig. 1: Information of human ovaries and quality control of scRNA-seq data.
From: Spatiotemporal transcriptomic changes of human ovarian aging and the regulatory role of FOXP1
(a) H&E staining of ovaries from women in young, middle and old groups for scRNA-seq and ST-seq (n = 15). (b) Percentages of mitochondrial genes detected in each sample. Box-and-whisker plots (minimum, 25th percentile, median, 75th percentile, maximum). (c) Left: numbers of unique molecular identifiers (UMIs) (upper) and genes (lower) detected in each cell. Right: UMIs counts and expressed genes are shown by three groups. (d) The number of expressed UMIs (nUMI) and genes (nGene) in different cell types. (e) UMAP plot showing the eight ovarian cell clusters in each sample. (f) UMAP plot showing all ovarian cell clusters. (g) Top three markers of each cell type shown as expression quantity (circle size) and mean expression (color). (h) UMAP cluster map of each slide showing expression of marker genes. (i) Bar plot showing the cell numbers of different cell types and detected genes in human ovaries. (j) Immunofluorescence staining of human GCs markers (AMH and GSTA1) and oocytes markers (ZP3 and TUBB8). The experiment was repeated for three times.
