Extended Data Fig. 2: Primary SCs form young donors and LOH patients.
From: Targeting dysregulated phago-/auto-lysosomes in Sertoli cells to ameliorate late-onset hypogonadism
a, b, Flowchart overview of primary SC isolation, identification, and culture (nSC_Y = 16 ROI, nSC_LOH = 13). c, d, Representative image and quantification showing autophagic flux blockade in the SCLOH samples (nSC_Y, SC_LOH = 15 ROI).e, The flow cytometry pattern of SC with mRFP-eGFP-LC3 transgene. f, The flow cytometry of green fluorescence intensity of mRFP-eGFP-LC3 SC between Y and LOH. Three individual patients for each group (d). The cells were derived from two individual patients for each group (b). Data are presented as mean ± s.e.m. Comparison by two-tailed unpaired t-test. Scale bars: 10 μm (b, c).
