Fig. 2: Regulation of DDX5 expression alters the progression of posttraumatic OA. | Nature Aging

Fig. 2: Regulation of DDX5 expression alters the progression of posttraumatic OA.

From: DDX5 inhibits hyaline cartilage fibrosis and degradation in osteoarthritis via alternative splicing and G-quadruplex unwinding

Fig. 2

a, Schematic of the experimental design. At 11 weeks of age, Ddx5loxP/loxP and Ddx5chon−/− male mice received five daily intraperitoneal injections of tamoxifen. One week after tamoxifen injection, mice underwent DMM surgery and were killed at 6 and 12 weeks postoperatively. SO&FG staining of the knee joint sections from Ddx5loxP/loxP and Ddx5chon−/− mice is shown. b, Corresponding OARSI and synovitis scores using histological sections (n = 8 mice per group). c, SO&FG staining of knee joint sections from Ddx5loxP/loxP and Ddx5chon−/− mice at 18 and 36 weeks. d, Quantification of the SO&FG staining area (red) and synovitis score using histological sections (n = 3 in 36-week-old Ddx5loxP/loxP mice, n = 4 mice in the other groups). e, Representative joint sections from mice with DMM injected intra-articularly with AAV2-NC or AAV2-Ddx5, stained with SO&FG. f, OARSI and synovitis scores (n = 6 mice per group). a,c,e, Scale bars, 100 µm. All data are expressed as the mean ± s.e.m. b,d,f, A two-way analysis of variance (ANOVA) with Šidák’s correction was conducted.

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