Extended Data Fig. 3: Additional compositional analysis of snRNA-seq clusters. | Nature Aging

Extended Data Fig. 3: Additional compositional analysis of snRNA-seq clusters.

From: Cell type mapping of inflammatory muscle diseases highlights selective myofiber vulnerability in inclusion body myositis

Extended Data Fig. 3

a Box plots showing compositional analysis of all identified cell-types within our dataset shown in Fig. 1c-e. Type 1 MN: p(CTRL-IBM) = 9.3 × 10−4, p(CTRL-IMNM) = 9.1 × 10−3; Type 2 MN: p(CTRL-IBM) = 6.1 × 10−3, p(IMNM-IBM) = 6.1 × 10−3; Reactive MN: p(CTRL-IBM) = 6.5 × 10−3; Damaged MN: p(CTRL-IBM) = 2.4 × 10−3, p(CTRL-IMNM) = 1.6 × 10−2, p(IMNM-IBM) = 1.6 × 10−2; FAPs: p(CTRL-IBM) = 3.7 × 10−3; T cells: p(CTRL-IBM) = 1.1 × 10−2; DC: p(CTRL-IBM) = 5.9 × 10−3, p(IMNM-IBM) = 6.1 × 10−3; MΦ: p(CTRL-IBM) = 3.7 × 10−3, p(IMNM-IBM) = 6.1 × 10−3. b Additional box plots showing compositional analysis of myonuclei in CTRL, IMNM, and IBM. Satellite cells: p(CTRL-IBM) = 6.2 × 10−2. c Additional box plots showing compositional analysis of immune cells in CTRL, IMNM, and IBM. LAMP3+ DC: p(CTRL-IBM) = 2.4 × 10−2, p(IMNM-IBM) = 2.2 × 10−2; TH cells: p(CTRL-IBM) = 1.8 × 10−2; Treg cells: p(CTRL-IBM) = 2.1 × 10−2, p(IMNM-IBM) = 2.1 × 10−2; NK cells: p(IMNM-IBM) = 1.2 × 10−2; Plasma cells: p(CTRL-IBM) = 9.2 × 10−2, p(IMNM-IBM) = 9.2 × 10−2. d Additional box plots showing compositional analysis of endothelial-stromal cells in CTRL, IMNM, and IBM. a-d (CTRL, n = 7; IMNM, n = 4; IBM, n = 8); Box plots show median and interquartile range (IQR) of cell/nuclei type proportions, with whiskers extending to the largest and smallest values within 1.5 × IQR range. Two-tailed pairwise Wilcoxon rank-sum tests with Benjamini-Hochberg correction to account for multiple comparisons or Tukey’s HSD tests were performed here between conditions.

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