Extended Data Fig. 3: DNA methylation, chromatin accessibility and transcriptional profiles of p14/p16 cell cycle regulator genes.
From: Conserved epigenetic hallmarks of T cell aging during immunity and malignancy
(a) Schematic cartoon of cell cycle regulator pathway related to p14/p16. Adapted from Sharpless and Sherr, Nature Reviews Cancer 2015. (b) DNA methylation and ATAC seq tracks showing the chromatin accessibility changes of DMR regions in the CDKN2A/2B locus. (c) Summary graphs for RNAseq transcript levels of CDKN2A and CDKN2B products. n = 3 biologically independent samples. Error bars represent standard deviation. (d) ATACseq profiles of Mdm2, Rb1 and Cdk6 loci. (e) Gene expression bar plots comparing Dnmt3a KO and Rosa KO (control). n = 2–5 biologically independent samples. Error bars represent standard deviation. (f) DNA methylation level plots showing Dnmt3a regulated gene body changes on Cdkn2a/2b.
