Extended Data Fig. 9: CD38 inactivation prevents HSC aging.
From: An NAD+-dependent metabolic checkpoint regulates hematopoietic stem cell activation and aging
a-h. Comparison of HSCs isolated from 24-month-old WT and CD38 KO mice. A, B. RNA-sequencing analyses of enriched HSCs. Volcano plot summarizing the differentially expressed genes (Red: upregulated in CD38 KO. Blue: downregulated in CD38 KO. P adj<0.1. Benjamini-Hochberg procedure). Genes related to ribosomal proteins are labeled (A). Gene ontology analysis for the biological functions of differentially expressed genes (B). n = 2. C, D. Flow cytometry analyses of TMRM (p = 0.0076) (C) and ROS (p = 0.0058) (D) staining. n = 10 (C). n = 10 and 12 (D). E. Quantitative real-time PCR analysis of HSP60 (p = 0.0022). n = 3. F-H. Staining for 7-AAD (p = 0.0029) (F), activated caspase 1 (p = 0.0254) (G), and Ki-67 (p = 0.1974) (H) were analyzed by flow cytometry. n = 6 (F). n = 6 and 5 (G). n = 5 and 6 (H). Data are presented as mean values +/- SE. *: p < 0.05. **: p < 0.01. ns: p > 0.05. One-sided student’s t test.
