Extended Data Fig. 1: CD38 is not required for HSC maintenance under homeostatic condition at young age.
From: An NAD+-dependent metabolic checkpoint regulates hematopoietic stem cell activation and aging
a-i. Comparison of 3-month-old WT and CD38 KO mice. A. Gating strategy for HSCs. Enriched HSCs: Lin−c-Kit+Sca1+. Highly enriched HSCs: Lin−c-Kit+Sca1+CD150+CD48−. B, C. The number of enriched (p = 0.4210) (B) and highly enriched (p = 0.4955) (C) HSCs in the bone marrow were analyzed via flow cytometry. n = 6. D, E. Ki-67 (p = 0.2928) (D) and 7-AAD (p = 0.2349) (E) staining of HSCs were analyzed via flow cytometry. n = 6. F. Lineage differentiation in the peripheral blood was analyzed via flow cytometry. MNCs, mononuclear cells (p = 0.4013). n = 5 and 6. G, H. Complete blood count analyses (G, p = 0.4174; H, p = 0.1768). n = 6. I. Bone marrow cellularity normalized by body weight (p = 0.0718). n = 6 and 5. Data are presented as mean values +/- SE. ns: p > 0.05. One-sided student’s t test.
