Extended Data Fig. 3: CD38 regulates Ca2+ signaling and mitochondrial activity upon HSC proliferation.
From: An NAD+-dependent metabolic checkpoint regulates hematopoietic stem cell activation and aging
a–c. Flow cytometry analyses of Rhod-2 (p = 0.0049) (A), TMRM (p = 0.0400) (B), ROS (p = 0.0497) (C) staining of HSCs isolated from 3-month-old WT and CD38 KO mice 16 hours post pIpC treatment. n = 5 and 6 (A, C). n = 5 (B). d. Gating strategy. e, f. Flow cytometry analyses of TMRM (p = 0.0265) (E) and ROS (p = 0.0137) (F) staining of CD38high and CD38low HSCs isolated from 3-month-old WT mice cultured for 18 hours in the presence of cytokines. n = 6 and 5 (E). n = 6 (F). Data are presented as mean values +/- SE. *: p < 0.05. **: p < 0.01. One-sided student’s t test.
