Fig. 7: Fenofibrate effectively restores tissue homeostasis in aged mice.
a, Experimental setup for fenofibrate treatment. Aged mice (23 months old) were treated with vehicle or fenofibrate (A-vehicle, A-fenofibrate), and then the senescent phenotypes were assessed. b,c, Gene expression of senescence and SASP markers in liver (n = 4 mice) (b) and brain (n = 4 mice for Y-vehicle; n = 3 mice for other groups) (c) of aged mice treated with fenofibrate. Color bars are the heatmap of relative mRNA expression. d,e, Protein expression of p21 and γH2A.X in liver and muscle (d) and their quantitative data (e) (n = 3 mice). f,g, Immunofluorescence detection of γH2A.X in liver and brain (f) and their quantitative data (g) (scale bar, 50 μm; n = 4 mice; 5–6 images per mouse). h, Phosphorylation levels of insulin signaling in liver (upper left) and muscle (upper right), and their quantitative analysis (bottom) (n = 3 mice). i,j, Representative µCT images (i) and quantitative analysis of bone volume/tissue volume (BV/TV) (j) (n = 4 mice). k, Hanging endurance (n = 4 mice). l,m, Immunofluorescence detection of GluR-1 in brain (l) and quantification of GluR-1 foci (m) (scale bar, 50 μm; n = 4 mice; 5–6 images per mouse). n, Novel object recognition (n = 4 mice). Statistical differences were determined by one-way ANOVA followed by Tukey’s multiple comparison test. mo, months; Rel. fold, relative fold; T.Ar, total area.
