Extended Data Fig. 1: Follicles accumulate age-related abnormalities.
From: Rejuvenation of aged oocyte through exposure to young follicular microenvironment
a,b, Representative images of Ki-67 staining in ovarian sections (a). F-actin was stained with phalloidin. Scale bar, 50 μm. Quantitative analysis of the percentage of Ki-67-positive cells per follicle is shown in (b). n = 31 (young), 25 (aged) follicles. c, Quantification of γH2AX foci in GCs from follicles in ovarian sections. n = 37 (young), 38 (aged) follicles. d-f, CM-H2DCFDA staining in isolated oocyte-GC complexes (d). Scale bar, 30 μm. Scatter plots (e) show the correlation between ROS levels in GCs and oocytes (simple linear regression and two-tailed analysis). Gray areas around fit lines indicate 95% confidence intervals, with Pearson’s correlation coefficient (r). Comparison of ROS intensity in young and aged oocytes or GCs is shown in (f). n = 76 (young), 43 (aged). 2-month-old (young) and 14-month-old (aged) mice were used in (b, c, e, f). Box plots in (b, c, f) show mean (black square), median (center line), quartiles (box limits), and 1.5× interquartile range (whiskers). Box plots inside the violins in (f) show mean (black circle), quartiles (box limits), and 1.5× interquartile range (whiskers). Two-tailed unpaired t-tests for (b, c, f). P value: ****P < 0.0001, ***P < 0.001. Exact P values are in the Source Data. Data are from at least three independent experiments.
