Extended Data Fig. 6: Eliminating H3K27ac impairs murine uterine receptivity.
From: Endometrial aging is accompanied by H3K27ac and PGR loss
a, Blastocysts retrieved from the CON and A485 groups. b, NANOG (inner cell mass marker) and CDX2 (trophectoderm marker) IF staining in blastocysts. c, The number of cells per blastocyst and the percentage of inner cell mass cells. d, Blastocysts were transplanted into sham pregnant mice and the number of implantation sites were measured on day 5. e, The percentage of Ki67-positive cells in uterine epithelial and stromal cells (n = 3). f, The H-score of PGR and ERα in the uterus on day 4 (n = 3). g, Relative protein levels of PGR and ERα in the uterus on day 4 (n = 3). h, PCA plot of murine uterine RNA-seq data (n = 4). i, Volcano plot illustrating gene expression changes between the CON and A485 murine uterus (|log2FC| > 1, P.adj < 0.05). j, Heatmap showing gene expression of DEGs between the CON and A485 murine uterus. k, Pathway enrichment analysis of downregulated DEGs in the A485 murine uterus. l, Pathway enrichment analysis of upregulated DEGs in the A485 murine uterus. Statistical analysis was performed by two-sided unpaired Student’s t-test or Mann–Whitney U rank-sum test. Data are presented as mean ± s.d. All replicates were biological replicates. CON, control; FC, fold change; ns, not significant; P.adj, adjusted P value; PC, principal component; PCA, principal component analysis.
