Fig. 4: H3K27ac and PGR exhibit correlated genomic occupancies and coordinated aging-related depletion.
From: Endometrial aging is accompanied by H3K27ac and PGR loss
a, Heatmaps of H3K27ac and PGR signals in young mid-secretory endometrial stromal cells at H3K27ac and PGR peaks in the young group. b, Venn diagram illustrating the overlap between genes marked by H3K37ac and PGR. c, The H3K27ac and PGR signals in young mid-secretory endometrial stromal cells at the euchromosome and X chromosome. d, One-kilobase plots showing the correlation between H3K27ac and PGR signals in human mid-secretory endometrial stromal cells. e, Motif analysis of H3K27ac peaks in young mid-secretory endometrial stromal cells. f, Co-IP assays showing the interaction between p300 and PGR in the human endometrium. g, Heatmaps of H3K27ac and PGR signals in human mid-secretory endometrial stromal cells at differentially binding peaks of H3K27ac and PGR between the two groups. h, Venn diagram illustrating the overlap between genes with H3K27ac and PGR loss. i, H3K27ac and PGR signals in mid-secretory endometrial stromal cells at selected genes. hg38 coordinates are shown. j, Protein levels of FoxO1, HOXA10 and HAND2 in mid-secretory endometrial stromal cells (n = 4). k, Relative protein levels of FoxO1, HOXA10 and HAND2 in mid-secretory endometrial stromal cells (n = 4). Statistical analysis was performed by two-sided unpaired Student’s t-test. Data are presented as mean ± s.d. l, GO enrichment analysis of genes with H3K27ac and PGR loss. m, KEGG enrichment analysis of genes with H3K27ac and PGR loss. All replicates were biological replicates. GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; mid-aged, middle-aged; P.adj, adjusted P value; Pro, proliferative phase; Sec, secretory phase; TES, transcription end site; WB, western blot.
