Extended Data Fig. 3: Genome-wide H3K27ac signals in mid-secretory endometrial stromal and epithelial cells of young and middle-aged patients.
From: Endometrial aging is accompanied by H3K27ac and PGR loss
a, H3K27ac immunofluorescence (IF) staining in the endometrium of different hormonal stages. Scale bar: 50 μm. b,c, H3K27ac in the human proliferative and mid-secretory endometrium (n = 5). Statistical analysis was performed by two-sided unpaired Student’s t-test. Data are presented as mean ± s.d. d, Schematic design of H3K27ac CUT&Tag in mid-secretory endometrial stromal and epithelial cells. e,f, Consistent H3K27ac signals between biological replicates (n = 3). g, Heatmaps of the H3K27ac signal in human mid-secretory endometrial stromal cells around gene bodies. h, Heatmaps of the H3K27ac signal in human mid-secretory endometrial epithelial cells at H3K27ac peaks in the young group. i, Volcano plot illustrating H3K27ac differences between young and aging mid-secretory endometrial epithelial cells. Red and blue points represent peaks that gain and lose H3K27ac in aging epithelial cells. j, The genomic distribution of H3K27ac peaks in human mid-secretory endometrial epithelial cells. k, Pathway enrichment analysis of genes marked by H3K27ac in young mid-secretory endometrial stromal cells. All replicates were biological replicates. FC, fold change; mid-aged, middle-aged; P.adj, adjusted P value; PC, principal component; PCA, principal component analysis; Pro, proliferative phase; Sec, secretory phase; TES, transcription end site; UTR, untranslated region.
