Extended Data Fig. 6: Analysis of single-nucleus sequencing from hypodermal DAF-2-AID worms treated with or without auxin.
From: Body-to-brain insulin and Notch signaling regulates memory through neuronal CREB activity
(a) Euclidean distance of each neuron’s mean Control (no auxin) vector and mean hypodermal DAF-2(-) vector. (b) UMAP of all nuclei color coded by sample type and biological replicate. (c) Number of nuclei by genotyping. (d) Number of UMIs per cell density. (e) Number of genes per cell density. (f) Number of nuclei relative to the number of genes auxin: minima 1.83, maxima 2.81, centre 2.48, bounds of box and whiskers 2.40, 2.57 and percentile (10 – 2.35) (90 – 2.64). Control: minima 1.83, maxima 2.78, centre 2.49, bounds of box and whiskers 2 39, 257and percentile (10 –2.31) (90 –2.63). Representative graph for n = 2 biological replicates for single-nucleus sequencing. (g) Number of genes relative to the number of UMIs. (h) Genes per UMI per cell density. (i) Hypodermal DAF-2(-) upregulated GNAQ-induced-genes in select neurons.
