Extended Data Fig. 10: Individual data of dead reckoning behavior tests and open field test upon chemogenetic inhibition.
a-d, Individual data of dead reckoning navigational tests from control (mCherry, n = 7 mice) and experimental (hM4Di, n = 8 mice) groups, showing search time (a), return time (b), heading angle (c), and error (d) from the same mice when treated with saline (left) and CNO (right). Bar charts displayed showed the mean. Paired two-tailed t-test. *P < 0.05, **P < 0.01. e-h, Performance of mice in both control (mCherry, n = 7 mice) and experimental (hM4Di, n = 8 mice) group showed no difference in open field test upon administration of saline and CNO, precluding the side effect of chemogenetic manipulation in locomotion. (e) Left, average speed of mice in control (Saline vs CNO = 8.61 ± 0.75 vs 9.32 ± 0.67 cm/s) and experimental group (saline vs CNO = 8.40 ± 0.60 vs 8.33 ± 0.82 cm/s). Middle, total distance of mice in control (Saline vs CNO = 51.56 ± 4.48 vs 55.90 ± 4.00 cm) and experimental group (Saline vs CNO = 50.42 ± 3.61vs 49.99 ± 4.96 cm). Right, time in center of mice in control (Saline vs CNO = 47.68 ± 7.69 vs 44.65 ± 6.31 s) and experimental group (Saline vs CNO = 42.09 ± 5.80 vs 37.48 ± 6.07 s). Bar chat showed the mean. Box and whisker plots displayed all sample points, with boxes indicating the interquartile range, central lines indicating the median and lower/upper whiskers extend to the minimum/maximum values. (f) Representative traces and heat maps in open field test from a mouse expressing chemogenetic vector hM4Di after treating with saline (left) and CNO (right). Individual comparison of total travel distance (g) and time spent in center area (h) in open field tests from the same mice treated with saline (left) or CNO (right) in both mCherry-expressing control group and hM4Di-expressing experimental group. Bar charts showed the mean of data. Statistics were tested by paired two-tailed t-test. Ctrl, n = 7 mice; hM4Di, n = 8 mice.
