Fig. 3: Metabolomic differentiation of PCOS in rural versus urban environments.
From: Comparing the metabolomic landscape of polycystic ovary syndrome within urban and rural environments
a PLS-DA scatter plot distinguishing Rural-PCOS (green) and Urban-PCOS (purple) groups along two principal components representing the most significant variance within the dataset. b OPLS-DA score plot enhances the metabolite-based separation between the groups, with T-scores depicting the discriminatory power. c VIP scores from the PLS-DA model rank the metabolites by their contribution to the group differentiation, with higher scores indicating greater importance. d The permutation test validated the PLS-DA model, with observed R2 and Q2 values far exceeding those from randomly permuted data, underscoring model reliability. e The feature importance plot displays the magnitude and reliability of each metabolite’s contribution to the model. f SAM plots for significant differential expression of metabolites between groups at a specified delta, demonstrating robust statistical significance with a low false discovery rate.
