Extended Data Fig. 10: LC-MS analysis of ACP-C-FkbH (GloB) and GloD active site mutagenesis. | Nature Synthesis

Extended Data Fig. 10: LC-MS analysis of ACP-C-FkbH (GloB) and GloD active site mutagenesis.

From: Discovery and biosynthetic pathway analysis of cyclopentane–β-lactone globilactone A

Extended Data Fig. 10

a LC-MS analysis of the one-pot enzymatic total biosynthesis of 3. b Mass spectrometric analysis of production of acylated ACP (12). Additional peaks marked by asterisks represent adventitious gluconylation (+178 Da) of N-terminal His6-tag during expression of the recombinant protein in E. coli.

Source data

Back to article page