Extended Data Fig. 3: Characterization of myeloid-cell subsets in RLNs and the diseased arterial wall.
a, Heatmap shows the top 10 DEGs in each myeloid-cell subtype. b, Marker combinations used to define 8 myeloid-cell subtypes are listed in Violin plots: subset 1, CD11b+ cDC; subset 2, CD11c− DC; subset 3, CD8a+ cDC; subset 4, pDC; subset 5, granulocytes including basophils and neutrophils; subset 6, Lyve1+ tissue-resident like macrophages; subset 7, monocyte/macrophages; subset 8, Trem2hi macrophages. Each dot represents one cell; Ccr7 (chemokine receptor involving in homing of T cells and DCs to lymph nodes), Ccl9 (chemokine or macrophage inflammatory protein-1 gamma attracts DCs); Xcr1 (chemokine receptor mediating leukocyte migration in response to inflammatory mediators), SiglecH (inhibits pDCs inflammatory responses), Trem2 (mainly expressed by macrophages with potential immunosuppressive activities), Ly6c2 (a cell surface glycoprotein mainly expressed by monocyte-macrophages and pDCs), Lyve1 (marker for lymphatic vessels and tissue-resident macrophages with hyaluronan transport activities), S100a8 (marker for granulocytes with calcium- and zinc-binding activities). c, Column graph of numbers of three major myeloid-cell subgroups (Y-axis) in each of four tissues (X-axis). Percentages of basophils/neutrophils, DCs, and macrophages are displayed in each column. d, Percentages of each DC subset per total DCs in WT RLNs, Apoe-/- RLNs, ATLOs and plaques. The total number of DCs analyzed in each tissue is shown in the inner circle. e, Percentages of each monocyte/macrophage subset per total macrophages in each tissue. The total number of cells analyzed in each tissue is shown in the inner circle.
