Extended Data Fig. 8: Dysregulation of costimulatory- and checkpoint inhibitor-related transcripts in APCs in ATLOs and plaques but not in RLNs of WT or Apoe-/- mice. | Nature Cardiovascular Research

Extended Data Fig. 8: Dysregulation of costimulatory- and checkpoint inhibitor-related transcripts in APCs in ATLOs and plaques but not in RLNs of WT or Apoe-/- mice.

From: Pairing of single-cell RNA analysis and T cell antigen receptor profiling indicates breakdown of T cell tolerance checkpoints in atherosclerosis

Extended Data Fig. 8

a, Myeloid-cell-derived costimulatory genes (Cd80, Cd86, Cd83, Cd40) and checkpoint inhibitor genes (Cd274/PD-L1, Pdcd1lg2/PD-L2, Fas, Icosl, Lgals3, Cd200) in CD45+ leukocytes in four tissues. Expression was normalized: Expression = (Average gene expression per cell type)*(Percentage of cell type per CD45+ leukocytes). b, Myeloid-cell-derived checkpoint inhibitor genes (Cd274/PD-L1, Pdcd1lg2/PD-L2, Fas, Icosl, Lgals3, Cd200) and costimulatory genes (Cd80, Cd86, Cd83, Cd40) per CD45+ leukocytes between WT blood vs Apoe-/- blood. c, The average expression of costimulatory genes and checkpoint inhibitor genes by different myeloid-cell subsets. d,e, Violin plots show Cd274 and Lgals3 gene expression by different myeloid-cell subsets. Each dot represents one single cell. Two-sided Kruskal-Wallis rank sum test was used to analyze the differences among groups.

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