Fig. 5: Delineation of plaque-inducible T cell transcript profiles. | Nature Cardiovascular Research

Fig. 5: Delineation of plaque-inducible T cell transcript profiles.

From: Pairing of single-cell RNA analysis and T cell antigen receptor profiling indicates breakdown of T cell tolerance checkpoints in atherosclerosis

Fig. 5

a, Schematic of experimental design. b, Heat maps of DEGs of pooled T cells carried identical TCRαβ pairs in different tissues. Each row represents one individual T cell. n = 28 T cells in plaques, 27 T cells in ATLOs and 26 T cells in Apoe−/− RLNs. Two-sided Wilcoxon rank-sum test was used to identify DEGs and significant DEGs were defined by adjusted P value < 0.05. c, Heat maps of plaque-inducible genes in three individual T cell clones. T cells with identical TCR sequences from different tissues were compared. The CDR3 aa sequences of the paired TCRα/β pairs are listed on top of the heat maps. d, Violin plots showed Cxcr6, Lgals1, S100a6 and Reep5 gene expression by different T cell subsets. Each dot represents one cell. Kruskal–Wallis rank-sum test with Benjamini–Hochberg correction was used to analyze the differences among groups.

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