Extended Data Fig. 4: Effect of Type I IFN on MerTK+ macrophage in vitro. | Nature Cardiovascular Research

Extended Data Fig. 4: Effect of Type I IFN on MerTK+ macrophage in vitro.

From: ATF3 coordinates the survival and proliferation of cardiac macrophages and protects against ischemia–reperfusion injury

Extended Data Fig. 4

a. Schematic representation of the in vitro experimental design. BMDMs obtained from WT and ATF3-CKO mice treated with rGAS6 for 4 days before hypoxia/reoxygenation, followed by GeneChip transcriptome analysis. b. Relative mRNA expression of randomly selected type I IFN-related genes in BMDMs obtained from ATF3-CKO and WT mice after HR (n = 6 mice per group). c–f. Quantification or representative images of EdU+ (c), Ki67+ (d), Annexin V+ (e), and TUNEL+ (f) cells in rGAS6-treated BMDMs stimulated with rIFN-α or rIFN-β (n = 6 biologically independent samples per group). Scale bar, 50 μm. Statistical significance was evaluated using two-tailed unpaired t tests (b), and one-way ANOVA analysis followed by Tukey’s multiple comparisons test (c–f). All data are presented as mean ± SEM.

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