Extended Data Fig. 1: Effects of CCL17 deficiency on atherosclerosis and Treg numbers.

(a) Experimental scheme of Apoe^−/− or Apoe^−/−Ccl17^e/e mice fed a Western-diet (WD) for 12 weeks; (b) Representative images and quantification of lesion area of Apoe^−/− (n = 16) or Apoe^−/−Ccl17^e/e (n = 14) mice measured after Oil-Red-O staining (ORO) for lipid deposits in the aortic root. Scale bar = 500 µm; (c) Quantification of lesion area measured after ORO for lipid deposits in the thoraco-abdominal of Apoe^−/− (n = 13) or Apoe^−/−Ccl17^e/e (n = 14); (d) Atherosclerotic lesion size in aortic arches of Apoe^−/− (n = 15) or Apoe^−/−Ccl17^e/e (n = 12), as quantified after H&E staining; (e-g) Quantification of the percentage of lesional Mac2⁺ macrophages (e), smooth muscle α-actin (SMA)⁺ smooth muscle cells (SMCs) (f) and collagen content (g) of Apoe^−/− (n = 11) or Apoe^−/−Ccl17^e/e (n = 10) mice fed a WD for 12 weeks; (h-i) Representative dot plots and flow cytometric quantification of CD45⁺CD3⁺CD4⁺ CD25⁺FoxP3⁺ Tregs in para-aortic lymph nodes (LNs) (h) and spleen (i) of Apoe^−/− (h, n = 8; i, n = 11) or Apoe^−/−Ccl17^e/e (h, n = 8; i, n = 10) mice; (j) Gating strategy for CD45⁺CD3⁺ CD4⁺CD25⁺FoxP3⁺ Tregs in lymphatic organs; (k-l) flow cytometric quantification of CD45⁺CD3⁺CD4⁺CD25⁺FoxP3⁺ Tregs in axillary (k) and inguinal LNs (l) of Apoe^−/− (k, n = 11; l, n = 10) or Apoe^−/−Ccl17^e/e (k, n = 10; l, n = 9) mice; (a-l) Data represent mean ± SEM. Two-sided P values as indicated and analyzed by unpaired Student’s t-test.

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