Extended Data Fig. 5: Fibrinogen binds to sTLT-1 in presence of HMB-001.
Influence of a) Buffer, b) HMB-001, and c) parent TLT-1 Ab (pTLT-1) on the ability of fibrinogen to bind to sTLT-1 was probed using SPR at 25 °C and pH 7.4 (n = 2) using the ABA injection strategy. As shown in the SPR assay setup (d), sTLT-1 was captured using anti-His Ab. Solution A consisted of either running buffer or 1 μM HMB-001 or 1 μM pTLT-1 Ab. Solution B consisted of fibrinogen concentration series in presence of running buffer or 1 μM HMB-001 or 1 μM pTLT-1 Ab. Coloured lines represent different fibrinogen concentrations starting from the highest concentration of 1000 nM (purple), 500 nM (yellow), 250 nM (pink), 125 nM (green), 62.5 nM (orange), and 0 nM (blue). Sensorgram from both runs are overlaid and shown below.
