Extended Data Fig. 4: Aβ staining in dura mater is associated mainly with bridging veins. | Nature Cardiovascular Research

Extended Data Fig. 4: Aβ staining in dura mater is associated mainly with bridging veins.

From: Sustained meningeal lymphatic vessel atrophy or expansion does not alter Alzheimer’s disease-related amyloid pathology

Extended Data Fig. 4

a-h, Whole-mount stainings of dorsal dura mater. BrV, bridging vein; dBV, dural blood vessel; dLV, dural lymphatic vessel. SSS and BrVs connecting to it are outlined in all panels by the white dashed line. a-f, Representative confocal images of DAPI (blue), endomucin (cyan), D54D2 (yellow), and LYVE1 (magenta) staining (a-c) and DAPI (blue), podocalyxin (cyan), D54D2 (yellow), and PROX1 (magenta) staining (d-f) in old APdE9 mouse (22-month-old non-treated female). White arrows indicate Aβ staining associated with BrV. White arrowheads indicate dLVs. g-h, Representative stereomicroscope images of podocalyxin (cyan), SMA (magenta), and D54D2 (yellow) staining in adult APdE9 mouse (9,5-month-old male) (g) and podocalyxin (cyan), BMX (magenta, stained with RFP), and vWF (yellow) staining in adult BmxCreERT2Rosa26LSL-TdTomato mouse (13-month-old male) (h). BrVs connecting to SSS are marked with yellow arrowheads. Data shown are representative of minimum n = 3 for every staining. Scale bars: 100 µm (a-f), and 500 µm (g, h).

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