Extended Data Fig. 1
From: Kupffer cells dictate hepatic responses to the atherogenic dyslipidemic insult
a, Schematic diagram of the APOE cKO and D374Y alleles. b, Enzyme-linked immunosorbent assay for plasma hPCSK9 protein in inducible male and female D374Y mice (blue line) and their littermate controls (black line) before and at 3 and 10 days after tamoxifen dosing (D374Y littermate control day 0: n = 1, Day 3: n = 2 and Day10: n = 5, D374Y day 0: n = 6, Day 3: n = 3 and Day10: n = 6). c, Plasma total cholesterol measurements (D374Y littermate control day 0: n = 6, Day 3: n = 6 and Day10: n = 6, D374Y day 0: n = 3, Day 3: n = 5 and Day10: n = 6). d, Cholesterol measurements 24 hours after tamoxifen administration from inducible male and female D374Y mice, (n = 6 D374Y and n = 5 littermate control mice). e, Immunoblot for LDLR expression in liver of D374Y mice or littermate controls after 10 days following tamoxifen induction maintained on a chow diet (n = 3 D374Y and n = 2 control, where every n represents a different mouse). f, Representative hematoxylin and eosin (H&E) sections of liver from APOE cKO, D374Y and respective littermate control mice at 10 days chow-fed or 8 weeks HFD diet following tamoxifen administration. Arrows indicate possible immune cell infiltration. Black scale bars represent 200 μm. n = 2 vs 2 in each group (where every n represents a different mouse) g, Circulating levels of AST, ALT, albumin, globulin, bilirubin and A/G ratio in dyslipidemic APOE cKO and D374Y mice with littermate controls, 10 days post tamoxifen induction while maintained on a chow diet and after 8 weeks on a high fat diet (10 days post tamoxifen: n = 8 APOE cKO and n = 8 littermate control mice; n = 9 D374Y and n = 7 littermate control mice. 8 weeks post tamoxifen while on a high fat diet: n = 8 APOE cKO and n = 6 littermate control mice; n = 8 D374Y and n = 8 littermate control mice). h, Histology showing ORO staining of atherosclerotic lesions in the aortic root of dyslipidemic APOE cKO and D374Y mice with littermate controls after 8 weeks on a high fat (scale bar =100 μm n = 3 vs 3 in each group (where every n represents a different mouse). All plots are mean ± SEM. Statistical analysis was performed with mixed model 2-way ANOVA (b,c), two-sided t-test (d) or one-way ANOVA (g).
