Fig. 5: Stroke activates circulating neutrophils to release cytotoxic NETs, which induce B cell loss. | Nature Cardiovascular Research

Fig. 5: Stroke activates circulating neutrophils to release cytotoxic NETs, which induce B cell loss.

From: Stroke and myocardial infarction induce neutrophil extracellular trap release disrupting lymphoid organ structure and immunoglobulin secretion

Fig. 5

a, Schematic of the experimental paradigm for neutrophil mass spectrometry and proteomics analysis. Blood neutrophils were isolated 6 h after sham surgery or stroke to perform liquid chromatography–mass spectrometry analysis. b, PCA of neutrophil proteomics after sham or stroke (n = 4 mice per group). c, Volcano plot comparing the normalized protein abundance in blood neutrophils of stroke mice versus sham-operated mice. Red dots indicate significantly upregulated proteins, and blue dots indicate significantly downregulated proteins. Two-tailed t-test, P < 0.05 adjusted with the Benjamini–Hochberg method, FC > 1.5 or FC < 0.5. d, Relative plasma levels of citH3-DNA (n = 5−6 per group, sham versus stroke ****P < 0.0001, stroke versus stroke+DNase-I ***P = 0.0004, stroke versus stroke+anti-Ly6G ****P < 0.0001). e, NE-DNA complexes after sham+isotype antibody, stroke+isotype antibody, stroke+DNase-I treatment or stroke+anti-Ly6G antibody treatment (n = 5−6 per group, sham versus stroke, stroke versus stroke+DNase-I, stroke versus stroke+anti-Ly6G treatment ****P < 0.0001). f, Numbers of CD19+ B cells in intestinal PP in sham-operated+isotype antibody, stroke+isotype antibody and stroke+anti-Ly6G antibody-treated mice (n = 5−7 per group, sham versus stroke, stroke versus stroke+anti-Ly6G treatment ****P < 0.0001). g, Numbers of CD19+ B cells in PP in sham-operated Ly6gcreMcl1f/f mice, stroke Ly6gcre and Ly6gcreMcl1f/f mice (n = 4−6 per group, sham versus Ly6gcre **P = 0.0013, stroke Ly6gcre versus stroke Ly6gcreMcl1f/f **P = 0.0095). h, Quantification of plasma IgA amounts sham, stroke, stroke+DNase-I treatment, stroke+anti-Ly6G antibody treatment or stroke Ly6gcreMcl1f/f mice (n = 6−13 per group, stroke versus sham, stroke versus stroke+DNase-I, stroke versus stroke+anti-Ly6G antibody, stroke versus stroke Ly6gcreMcl1f/f ****P < 0.0001). Data represent mean ± s.d., Shapiro−Wilk normality and ordinary one-way ANOVA with Bonferroni’s multiple comparisons tests.

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