Extended Data Fig. 6: Validation, single cell RNA sequencing, and reference mapping of Arg1ZsGr mice after MI.
a, Representative 20x confocal images of ARG1 IHC in the infarct and remote zone of Arg1ZsGr mice 2, 7, 30 days after I/R. Quantification of IHC is displayed as the percentage of ZsGr+ cells that are ARG1+ZsGr+. N = 4 vs 4 vs 3. Sensitivity of Arg1ZsGr mice 2 days after I/R within the infarct, quantified as the percentage of ARG1+ cells that are ZsGr+ or ZsGr−. N = 4 vs 4. b, Representative 20x confocal images of VSIG4 IHC in the infarct and remote zone of Arg1ZsGr mice 2, 7, 30 days after I/R. Quantification of IHC is displayed as the percentage of ZsGr+ cells that are VSIG4+ZsGr+. N = 3 vs 4 vs 3. c, FACS gating strategy for isolation of ZsGr− and ZsGr+ monocytes and macrophages from Arg1ZsGr mice 2 and 30 days after I/R. d, Violin plots representing scRNAseq data post quality control. Cells with greater than 500 and less than 5000 genes (nFeature_RNA), read counts less than 20,000 (nCount_RNA), and proportion of transcripts mapping to mitochondrial genes less than 10 percent (prompt) were used. e, Arg1 expression in the ZsGr− and ZsGr+ libraries at day 2 and 30. f, Stacked bar graph representing the proportion of each cell population in the data split between ZsGr− and ZsGr+ cells 2 and 30 days after I/R. g, Heat map of mapping scores for each cell population in the Query (Arg1ZsGr) plotted against the Reference MI data set. h, Z-score profiles from the reference data set (Extended Data Fig. 2f) compared to each subpopulation in the Arg1ZsGr data set represented as a dot-plot. i, Reference UMAP plots split between ZsGr− and ZsGr+ libraries 2 and 30 days after I/R after mapping to reference MI data set. The reference MI scRNAseq data set was the same as the one displayed in Extended Data Fig. 2. P-values are determined using the ordinary one-way ANOVA using Tukey’s multiple comparisons test. For N, each data point is an individual mouse. Data are presented as mean ± SEM.
