Extended Data Fig. 10: FACS, validation, single cell RNA sequencing, and reference mapping of Arg1DTR mice after MI.
a, FACS gating strategy for the isolation of monocytes/macrophages/dendritic-like cells and comparison of Arg1-TDT expressing monocytes/macrophages between Control and Arg1DTR mice 3 days after I/R. b, Violin plots representing post-quality control scRNAseq data of monocytes/macrophages/dendritic-like cells in Control and Arg1DTR mice 3 days after I/R. Cells with between 500 and 5000 genes (nFeature_RNA), more than 500 and less than 20,000 read counts (nCount_RNA), and percentage of transcripts mapping to mitochondrial genes less than 10 percent (prompt) were used for downstream analysis. c, Reference UMAP plot of Control and Arg1DTR scRNAseq data after mapping to reference MI data set. The reference MI scRNAseq data set was the same as the one displayed in Extended Data Fig. 2. d, Heat map of mapping scores for each cell population inf the Query (Control and Arg1DTR) plotted against the Reference MI data set. e, Z-score profiles from the reference data set (Extended Data Fig. 2f) compared to each subpopulation in the Control and Arg1DTR data set represented as a dot-plot. f, IL-13, KC, LIX, and MIP2 protein levels in Control and Arg1DTR mice 3 days after I/R quantified via Luminex. Concentrations (pg/ml) are normalized to the amount of protein in the sample (µg). N = 6 vs 4 (each data point is an individual mouse). P-values are determined using a two tailed t-test assuming equal variance. Data are presented as mean ± SEM.
