Fig. 3: In- and ex vivo comparison of FA-ICG (vs ICG) accumulation in orthotopic U87-MG glioblastoma xenograft model.
From: Near-infrared fatty acid molecular probe for image-guided surgery of glioblastoma
a, b In- and ex vivo fluorescence accumulation of FA-ICG (a) compared to fluorescence accumulation of ICG (b) at 8 h after drug administration on IVIS® Spectrum. Higher accumulation of FA-ICG (compared to ICG) is demonstrated on the right side of the head and in the right (tumor-bearing) brain hemisphere. c, d Coronal thionine-stained brain sections demonstrate tumor presence in the right cerebral hemisphere in two different mouse sections (in dark violet). e NIR imaging demonstrates increased NIR fluorescence signal in the same brain slice on flatbed imaging (c, e show same slice), tumor presence co-localizes with NIR signal f Limited NIR fluorescence signal is observed in the ICG administered mouse, albeit slightly more in the tumor than in surrounding brain as compared with thionine staining (d), d, f show same slice. The sample size per experimental group described is n = 3. Error bars report on standard deviation. Statistical analyses were performed using a one-way ANOVA test followed by the Bonferroni–Dunn method for multiple mean comparison. Statistical significance was set at p < 0.05 (*< 0.05, **<0.01, ***<0.001).
