Fig. 4: NIR image-guided surgery of U87-MG glioblastoma tumor-bearing mice (FA-ICG vs ICG).
From: Near-infrared fatty acid molecular probe for image-guided surgery of glioblastoma
Intraoperative fluorescence imaging demonstrates accumulation of FA-ICG probe, left panel (b, d, f, h), when compared to ICG, right panel (c, e, g, i), on QUEST Spectrum® 2 image-guided surgery camera. a Schematic representation of the timeline of the experiment. b, c Transcutaneous fluorescence imaging demonstrates diffuse fluorescence signal in FA-ICG and ICG administered mice. d, e Transcranial fluorescence imaging demonstrates localized fluorescence signal (point or dot-shaped) in FA-ICG administered mouse (d) while more diffuse signal is observed in case of ICG (e). Notably, the brightfield image (e) shows more hemorrhage below the skull (and not clear tumor). f, g Higher intraparenchymal NIR signal (as shown in f and h) is demonstrated in the right cerebral hemisphere for FA-ICG, while in the ICG administered mouse (g and i) signal is observed in the middle (and the hemorrhage below the skull disappears). h, i Ex vivo imaging of the brain demonstrates considerably higher signal in the FA-ICG administered mouse than in the ICG administered mouse. The sample size per experimental group described is n = 3.
