Figure 3: Virus titers of FHM cells infected with TFV.

60 h after HepG2 cells were infected with TFV at an MOI of 10. (A) The cells were treated with 5mM MβCD, or (B) 200 μg/ml nystatin for 12 h. The supernatant of the cells was collected and the Virus titers were measured. (C) 60 h after HepG2 cells were infected with TFV at an MOI of 10, the cells were treated with 5 mM MβCD or not (NC). The supernatant of the cells was collected at different time point (0, 2, 4, 8 and 12 h) after treated and the Virus titers were measured. Y-axes represent Virus titers. (D) 60 h after FHM cells were infected with TFV at an MOI of 0.1, the cells were treated with 2 mM MβCD, or 100 μg/ml nystatin for 12 h. The supernatant of the cells was collected and the virus titers were measured. Y-axes represent absolute quantitative values of TFV gene orf096r. Asterisks indicate values that are statistically significant (p < 0.05).