Figure 8: Effects of GW on L-Arg induced enhancement of Ca²⁺ oscillations.

(A) After pretreatment with GW, bath-applied L-Arg (10 mM for 10 min) fails to enhance ACh-induced Ca²⁺ oscillations. Traces in Fig. 8Aa,b were recorded from the same cell. (B) Without pretreatment, bath-applied L-Arg enhances ACh-induced Ca²⁺ oscillations, and under this condition, the addition of GW also reduces L-Arg-induced enhancement of Ca²⁺ oscillations. Traces in Fig. 8Ba,b were recorded from the same cell. (C,D) GW significantly blocks L-Arg-induced enhancement of Ca²⁺ oscillations either with or without pretreatment of GW. Bar graphs represent averaged charge ± SEM. The number of cells tested is stated for each condition in parentheses. *Indicates p < 0.05, ***Indicates p < 0.001.