RAB-11-positive recycling endosomes participate in the establishment and maintenance of epithelial polarity. Zerial and colleagues carry out an in vivo image-based RNAi screen for factors that regulate recycling endosome positioning in Caenorhabditis elegans. They identify, among other candidates, PAR-5 as a key determinant of recycling endosome positioning and, thus, apicobasal polarity.
- Julia Franziska Winter
- Sebastian Höpfner
- Marino Zerial