Histone modifications have been implicated in many DNA transactions. Using a mini-chromosome isolation and mass spectroscopy technique applicable to specifically probing many DNA binding factors, the acetylation of histone residues surrounding an S. cerevisiae origin are now defined during the cell cycle, with further experiments implicating residues in efficient origin activation.
- Ashwin Unnikrishnan
- Philip R Gafken
- Toshio Tsukiyama
