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Advanced filters: Author: Chaoyi Qu Clear advanced filters

Improving polyketide biosynthesis by rescuing the translation of truncated mRNAs into functional polyketide synthase subunits

Genes encoding bacterial modular polyketide synthases (mPKSs) are usually longer than 10 kb and their mRNAs have high risk of being truncated. Here, the authors showed that they can enhance mPKS-based natural product biosynthesis by rescuing the translation of truncated mRNAs through PKS module splitting.

Yan Liu
Chaoyi Song
Hailong Wang
ResearchOpen Access17 Jan 2025
Nature Communications

Volume: 16, P: 1-15
Seamless site-directed mutagenesis in complex cloned DNA sequences using the RedEx method

RedEx achieves seamless genetic modification of large, highly repetitive, DNA targets by combining Redαβ-mediated linear–circular homologous recombination, ccdB counterselection and exonuclease-mediated in vitro annealing.

Ji Luan
Chaoyi Song
Hailong Wang
Protocols15 Jul 2024
Nature Protocols

Volume: 19, P: 3360-3388
Maximum oxygen consumption and quantification of exercise intensity in untrained male Wistar rats

Fei Qin
Yanan Dong
Jiexiu Zhao
ResearchOpen Access13 Jul 2020
Scientific Reports

Volume: 10, P: 1-8

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