Nature Search

Oblique line scan illumination enables expansive, accurate and sensitive single-protein measurements in solution and in living cells

Oblique line scan microscopy achieves nanoscale spatial and sub-millisecond temporal resolution across a large field of view, enabling improved and robust single-molecule biophysical measurements and single-molecule tracking in both cells and solution.

Amine Driouchi
Mason Bretan
Daniel J. Anderson
ResearchOpen Access18 Feb 2025
Nature Methods

Volume: 22, P: 559-568
Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue

Direct wavefront sensing with laser ‘guide stars’ is used in astronomy and microscopy to correct for optical aberrations. Wang et al.use near-infrared guide stars to extend this approach to the highly scattering mouse brain, allowing high-resolution fluorescence imaging at 700μm depth.

Kai Wang
Wenzhi Sun
Na Ji
ResearchOpen Access15 Jun 2015
Nature Communications

Volume: 6, P: 1-6
High-speed, low-photodamage nonlinear imaging using passive pulse splitters

Na Ji
Jeffrey C Magee
Eric Betzig
Research20 Jan 2008
Nature Methods

Volume: 5, P: 197-202
Image processing tools for petabyte-scale light sheet microscopy data

PetaKit5D offers versatile processing workflows for light sheet microscopy data including performant image input/output, geometric transformations, deconvolution and stitching. The software is efficient and scalable to petabyte-size datasets.

Xiongtao Ruan
Matthew Mueller
Srigokul Upadhyayula
ResearchOpen Access17 Oct 2024
Nature Methods

Volume: 21, P: 2342-2352
Erratum: High-speed, low-photodamage nonlinear imaging using passive pulse splitters

Na Ji
Jeffrey C Magee
Eric Betzig
Amendments and Corrections01 Feb 2009
Nature Methods

Volume: 6, P: 179
High-density mapping of single-molecule trajectories with photoactivated localization microscopy

Suliana Manley
Jennifer M Gillette
Jennifer Lippincott-Schwartz
Research13 Jan 2008
Nature Methods

Volume: 5, P: 155-157
Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules

The adaptor protein zyxin is known for its mechanosensing function in the maintenance of actin network. Here the authors show that zyxin is key to blood homeostasis and thrombosis by controlling the endothelial release of von Willebrand factor and the formation of actin scaffolds on exocytic granules.

Xiaofan Han
Pin Li
Jincai Luo
ResearchOpen Access03 Mar 2017
Nature Communications

Volume: 8, P: 1-11
A Cell Observatory to reveal the subcellular foundations of life

Imaging the 4D choreography of subcellular events in living multicellular organisms at high spatiotemporal resolution could reveal life’s fundamental principles. Yet extracting these principles from petabyte-scale image data requires fusing advanced light microscopy and cutting-edge machine learning models with biological insight and expertise.

Eric Betzig
Comments & Opinion07 Aug 2024
Nature Methods

Volume: 22, P: 646-649
Nanoparticle-based targeting of microglia improves the neural regeneration enhancing effects of immunosuppression in the zebrafish retina

Analysing microglial activity in a zebrafish model of photoreceptor degeneration shows the immunosuppressive effect of dexamethasone in vivo and that dexamethasone conjugation to dendrimer nanoparticles greatly enhances photoreceptor regeneration.

Kevin Emmerich
David T. White
Jeff S. Mumm
ResearchOpen Access18 May 2023
Communications Biology

Volume: 6, P: 1-14
3D ATAC-PALM: super-resolution imaging of the accessible genome

3D ATAC-PALM integrates ATAC with super-resolution imaging for nanoscale views of the accessible genome. When combined with FISH, protein fluorescence and genetic perturbation, the method enables investigation of accessible chromatin in situ.

Liangqi Xie
Peng Dong
Zhe Liu
Research16 Mar 2020
Nature Methods

Volume: 17, P: 430-436
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination

Light sheet microscopy using a scanned Bessel beam in combination with structured illumination or two-photon excitation reduces photobleaching and phototoxicity, improves axial resolution and allows isotropic three-dimensional imaging. The authors demonstrate performance of the method via fast volumetric subcellular imaging of several dynamic processes in single living cells.

Thomas A Planchon
Liang Gao
Eric Betzig
Research04 Mar 2011
Nature Methods

Volume: 8, P: 417-423
Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics

Hari Shroff
Catherine G Galbraith
Eric Betzig
Research13 Apr 2008
Nature Methods

Volume: 5, P: 417-423
Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells

Hayer et al. observe that collectively migrating endothelial cells extend rear VE-cadherin-rich membrane structures that are engulfed by follower cells, correlating spatially with the direction of movement.

Arnold Hayer
Lin Shao
Tobias Meyer
Research14 Nov 2016
Nature Cell Biology

Volume: 18, P: 1311-1323
Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues

Microscope imaging performance can be seriously degraded by optical inhomogeneities in biological samples. An adaptive optics approach using a spatial light modulator to divide the illumination wavefront into individually controllable subregions recovers near-diffraction–limited two-photon imaging performance in brain tissue.

Na Ji
Daniel E Milkie
Eric Betzig
Research27 Dec 2009
Nature Methods

Volume: 7, P: 141-147
High-density three-dimensional localization microscopy across large volumes

Lattice light-sheet and PAINT microscopy are combined to achieve low-background detection of dense molecular labels, yielding super-resolution localization microscopy images of intricate 3D structures within dividing cells and embryos.

Wesley R Legant
Lin Shao
Eric Betzig
Research07 Mar 2016
Nature Methods

Volume: 13, P: 359-365
Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments

Using structured illumination microscopy, Beach et al. and Hu et al. visualize the assembly of myosin II filaments in cells, describing a filament-partitioning mechanism, and long-range self-organization of filaments, respectively.

Jordan R. Beach
Kyle S. Bruun
John A. Hammer
Research23 Jan 2017
Nature Cell Biology

Volume: 19, P: 85-93
Applying systems-level spectral imaging and analysis to reveal the organelle interactome

Using confocal and lattice light sheet microscopy, the authors perform systems-level analysis of the organelle interactome in live cells, allowing them to visualize the frequency and locality of up to five-way interactions between different organelles.

Alex M. Valm
Sarah Cohen
Jennifer Lippincott-Schwartz
Research24 May 2017
Nature

Volume: 546, P: 162-167
Rapid adaptive optical recovery of optimal resolution over large volumes

Adaptive optics microscopy using a de-scanned, laser-induced guide star and direct wavefront sensing allows high numerical aperture diffraction-limited imaging of fine dynamic structures deep in the intact living zebrafish brain.

Kai Wang
Daniel E Milkie
Eric Betzig
Research13 Apr 2014
Nature Methods

Volume: 11, P: 625-628
Cytoskeletal actin patterns shape mast cell activation

Huw Colin-York et al. use advanced microscopy techniques to show that the cortical actin network within a model mast cell line undergoes a series of reorganizational events at the basal interface during activation. They find that actin patterns co-localize with zones of Arp2/3 nucleation and myosin-II activity accompanies network reassembly.

Huw Colin-York
Dong Li
Marco Fritzsche
ResearchOpen Access07 Mar 2019
Communications Biology

Volume: 2, P: 1-12
3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy

Liang Gao
Lin Shao
Eric Betzig
Protocols10 Apr 2014
Nature Protocols

Volume: 9, P: 1083-1101

Search

Oblique line scan illumination enables expansive, accurate and sensitive single-protein measurements in solution and in living cells

Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue

High-speed, low-photodamage nonlinear imaging using passive pulse splitters

Image processing tools for petabyte-scale light sheet microscopy data

Erratum: High-speed, low-photodamage nonlinear imaging using passive pulse splitters

High-density mapping of single-molecule trajectories with photoactivated localization microscopy

Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules

A Cell Observatory to reveal the subcellular foundations of life

Nanoparticle-based targeting of microglia improves the neural regeneration enhancing effects of immunosuppression in the zebrafish retina

3D ATAC-PALM: super-resolution imaging of the accessible genome

Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination

Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics

Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells

Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues

High-density three-dimensional localization microscopy across large volumes

Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments

Applying systems-level spectral imaging and analysis to reveal the organelle interactome

Rapid adaptive optical recovery of optimal resolution over large volumes

Cytoskeletal actin patterns shape mast cell activation

3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy

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